Paper published on bacterial fluorescent stain TramTO

Our article entitled “A Far-Red Fluorescent DNA Binder Enables Interaction Studies of Live Multidrug-Resistant Pathogens and Host Cells” has been accepted for publication by Angewandte Chemie, one of the leading journals in chemistry. This interdiscplinary work by the Schulte and Vazquez labs describes a new far-red bacterial fluorescent dye, TramTO, suitable for FACS and microscopy analysis of the interplay of live bacterial pathogens with their host cells. We show that TramTO labeling may replace bacterial fluorescent protein-tagging methods in macrophage uptake assays with the advantages of a less time-consuming labeling strategy and the applicability to bacteria hard to manipulate genetically. Exemplarily we use the TramTO method to study the interaction of primary human macrophages with multi-drugresistant Klebsiella pneumoniae, a major human health threat, which has received highest research priority by the World Health Organization.

Leon N. Schulte Benedikt Heinrich Harshavardhan Janga Bernd T. Schmeck Olalla Vazquez. A Far‐Red Fluorescent DNA Binder Enables Interaction Studies of Live Multidrug‐Resistant Pathogens and Host Cells. Angeandte Chemie 2018, https://doi.org/10.1002/anie.201804090

Press release: “Mit Farbstoff gegen überlebenskünstler”…

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