Our paper, revealing long non-coding RNA MaIL1 as a component of the TLR4-TRIF immune signaling pathway is now out in PNAS. We reveal MaIL1 as a critical component of type I interferon-mediated innate antibacterial defense in human macrophages. The paper also reports previously unknown subclasses of long non-coding RNAs (lncRNAs), co-sedimenting with different cellular protein machineries, and describes a new workflow for lncRNA mechanistic investigation.
A step-by-step protocol for our new long non-coding RNA gene knockout method has been deposited at protocols.io: http://dx.doi.org/10.17504/protocols.io.mmic44e
Check our recent publication introducing an improved Cas9-based method for silencing of long non-coding RNA genes: 'Cas9-mediated excision of proximal DNaseI/H3K4me3 signatures conferse robust silencing of microRNA and long non-coding RNA genes' Janga H, Aznaourova M, Boldt F, Damm K, Grünweller A, Schulte LN https://doi.org/10.1371/journal.pone.0193066